内容简介
爪蟾是生物学研究中的一种模式生物，在功能基因组、发育生物学、细胞生物学、 蛋白质组学和神经生物学研究中占有较重要的地位，在脊椎生物的早期发育研究中扮Development演着非常重要的角色。《爪蟾早期发育实验指南(影印版)》是冷泉港实验室出版社Early Deuelopment of Xenopus Laevis：A Laboratory Manual的影印本。书中的实验方案涵盖了爪蟾胚胎、成体的形态发生，爪蟾中基因表达操作，胚胎的获取和实验常规操作，微注射，免疫细胞化学，原位杂交，培养基和培养液等爪蟾早期发育研究的内容。每一个方案都经过专家的精心挑选和雕琢，实验设计严谨、准确、简洁、规范，可操作性强，值得称道。书中既包含了初学者需要了解的基础知识，也涵盖了资深研究者所需的细节。《爪蟾早期发育实验指南(影印版)》的版式设计侧重于方便读者使用，正文中穿插了丰富的图表作为实验设计的辅助说明，附录中还列出了爪蟾早期发育研究所需的重要资料如基本溶液、缓冲液的配方和配制方法等。《爪蟾早期发育实验指南(影印版)》适合于从事发育生物学、细胞生物学、基因组学、遗传学、分子生物学、药物设计和开发以及功能基因组学研究的相关教学科研人员以及相关学科的本科生、研究生参考使用。
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推荐理由
Early Development of Xenopuslaevis
By Hazel L. Sive, Whitehead Institute for Biomedical Research; Robert M. Grainger, University of Virginia, Charlottesville; Richard M. Harland, University of California, Berkeley.
Description
Amphibian embryos are supremely valuable in studies of early vertebrate development because they are large, handle easily, and can be obtained at many interesting stages. And of all the amphibians available for study, the most valuable is Xenopus laevis, which is easy to keep and ovulates at any time of year in response to simple hormone injections. Xenopus embryos have been studied for years but this is a particularly exciting time for the field. Techniques have become available very recently that permit a previously impossible degree of manipulation of gene expression in intact embryos, as well as the ability to visualize the results of such manipulation. As a result, a sophisticated new understanding of Xenopus development has emerged, which ensures the species continued prominent position among the organisms favored for biological investigation.
This manual contains a comprehensive collection of protocols for the study of early development in Xenopus embryos. It is written by several of the field most prominent investigators in the light of the experience they gained as instructors in an intensive laboratory course taught at Cold Spring Harbor Laboratory since 1991, As a result it contains pointers, hints, and other technical knowledge not readily available elsewhere.
This volume is essential reading for all investigators interested in the developmental and cell biology of Xenopus and vertebrates generally.
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目录
Preface
Acknowledgments
Chapter 1 Introduction
Chapter 2 Morphology of Xenopus Embryos and Adults
Chapter 3 Manipulating Gene Expression in Xenopus
Chapter 4 Equipment for Embryo Experiments
Chapter 5 Obtaining Embryos
Chapter 6 Preparing Embryos for Manipulation
Chapter 7 Embryo Perturbations
Chapter 8 Microinjection
Chapter 9 Fate Mapping and Lineage Labeling
Chapter 10 Microdissection
Chapter 11 Transgenesis in Xenopus Embryos
Chapter 12 Immunocytochemistry
Chapter 13 Whole-mount In situ Hybridization
Chapter 14 Histology
Appendix 1 Culture Media and Solutions
Appendix 2 Timing of Development of Xenopus laevis Embryos and Temperature Dependence
Appendix 3 Nucleic Acid Methods
Appendix 4 Cautions
Appendix 5 Suppliers
Appendix 6 Trademarks
Index
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