内容简介
近年来，植物生物技术有了飞速的发展，其应用领域益加广泛。本书是Introduction to Plant Biotechnology第二版的影印本，对植物的基因、基因组的组成，植物组织培养、重组DNA技术进行了全面翔实的阐述，与第一版相比，特别增加了体外突变发生、基因组学、生物信息学、基因转移方法和作物改良转基因技术等方面的内容。作者在生物技术和遗传学领域从事教学和研究20多年，在植物体外培养、基因转移、分子标记方面做出了杰出的成就，有丰富的经验，在写作本书时特别注重技术的应用，也提供了比较详细的实验室操作方案。
本书知识全面，内容丰富，集合了植物生物技术的基础知识和前沿进展，实用性强，适合相关学科的高年级本科生、研究生用作教材，也适合从事相关工作的科研技术人员参考使用。
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目录
Preface to the Second Edition
Preface to the First Edition
AbbreviatIOns
PART I：PLANT TISSUE CUL.TURE
1.INTRODUCTION
New technologies
Origin of biotechnology
History
2 LABORATORY ORGANIZTION
Washing facility
General laboratory and media preparation area
Transfer area
Culturing facilities
Light units
Green houses
Laboratory and pemonal safety
3.NUTRITlON MEDIUM
Facilities and equipment
Units for solution preparation
Media composition
Inorganic nutrients
Carbon and energy source
Vitamins
Growth regulators
Organic supplements
Gelling agent
pH
Protocol：General methodology for medium preparation
4.STERILIZATION TECHNIQUES
Preparation of sterile media，containers and small instruments
Steam sterilization
Units of energy and pressure
Dry sterilization
Filter sterilization
Ultra violet sterilization
Maintenance of aseptic conditions
AIcohoI sterilization
Flame sterilization
Sterilization of explant
ChemicaI sterilization
Protocols
Sterilization of seeds
Sterilization Of buds，leaf，stem，roots，tubers，scales，etc.
Sterilization of tissue for immature embryos，ovules
and flower buds for anther culture
5.TYPES OF CULTURE
CytodifferentIation
Organogenic differentiation
Types of culture
Seed culture
Embryo culture
Mature embryo culture
Immature embryo culture／embryo rescue
Applications of embryo culture
Callus culture
Organ culture
Nucellus culture
Application
Endosperm culture
Application
Cell culture
Protoplast culture
Protocols
Protocol for seed germination（Nicotiana）
Protocol for embryo culture（Cereals-wheat，maize，barley rice，etc.）
Protocol for embryo culture（Legumes-green gram，black gram，
french bean，soybean，etc.）
Protocol for callus induction（Nicotiana tabacum）
Protocol for callus induction（Cereals-wheat，rice，maize，barley,etc.）
6.MICROPROPAGATlON
Axillary bud proliferation approach
Meristem and shoot tip cultu re
Bud culture
Single node culture
Axillary bud method
OrganOgenesis
Organogenesis via callus formation
Direct adventitious organ formation
Embryogenesis
Advantages Of micropropagation
Problems associated with micrOprOpagatiOn
Protocols
Meristem and node culture Of potato（Solanum tuberosum）
Proliferation of axillary buds（Strawberry-Fragaria chiloensis）
OrganOgenesis-adventitious shoot formation
Organogenesis via callus formation（Nicotiana）
Organogenesis via callus formation（Cereals：wheat，barley,
maize，rice，etc.）
Embryogenesis（Carrot）
7.CELL SUSPENSION AND SECONDARY MEl-ABOLITES
Ivpes of suspension cultures
Batch culture
Continuous culture
Open continuous culture
CIosed continuous culture
Semi continuous culture
G rowth measu rements
Synchronization of suspension culture cells
Technique for single cell culture-Bergmann cell plating technique
Applications
Production of secondary metabolites
Morphological and chemicaI differentiation
Medium composition for secondary product formation
G rowth production patterns
EnvironmentaI factors
Selection of cell lines producing high amounts of a useful metabolite
Product analysis
Application
Problems associated with secondary metabolite production
Immobilized cell systems
Polymers for immobilization
Product release
Biotransformation
Protocols
Protocol for cell suspension culture（Nicotiana tabacum）
Protocol for cell suspension culture（Cereals-wheat，rice，
maize，barley,etc.）
8.IN VITRO PRODUCTION OF HAPLOIDS
Androgenic methods
Anther cultu re
Microspore culture
The various factors governing the success of androgenesis
Genotype
Physiological statuS of the donor plants
Stage of pollen
Pretreatment of anthers
Culture media
Process of androgenesis
The ploidy Ievel and chromosome doubling
Diploidization
Significance and uses of haploids
Problems
Gynogenic haploids
Factors affecting gynogenesis
Chromosome elimination technique for production Of haploids
in cereals（Barley and wheat）
Protocol
Protocol for anther culture of cereals（Rice，barley,wheat，etc.）
9.PROTOPLAST ISOLATION AND FUSION
Protoplast isolation
MechanicaI method
Enzymatic method
Physiological state of tissue and cell material
Enzymes
Osmoticum
Protoplast purification
Protoplast viability and density
Culture techniques
Culture medium
Environmental factors
Protoplast development
Cell walI formation
Growth，division and plant regeneration
Somatic hybridization
Protoplast fusion
Spontaneous fusion
Induced fusion methods
Treatment with sodium nitrate
Calcium ions at high pH
Polyethylene glycol method
Electrofusion
Mechanism of fusion
Identification and selection of hybrid cells
Chlorophyll deficiency complementation
Auxotroph complementation
Complementation of resistance markers
Use of metabolic inhibitors
Use Of visuaI characte ristics
Compound selection system
10.SoMACLONAL VARLATION
11.GERMPLASM STORAGE AND CRYOPRESERVATION
12.GENETIC MArERlAL
13.ORGANIZATION OF DNA AND GENE EXPRESSION
14.BASIC TECHNIQUES
15.GENE CLONING：Cutting and Joining DNA Molecules
16.GENE CLONING：Vectors
17.GENE CLONING：cDNA and Genomic Cloning and Analysis of
Cloned DNA Sequences
18. POLYMERASE CHAIN REACTlON
19.IN VITRO MUTAGENESlS
20.TRANSPOSON GENETIC ELEMENTS AND GENE TAGGING
21.GENE ISOLATION
22.MOLECULAR MARKERS AND MARKER.ASSlSTED SELECTION
23. GENE TRANSFER IN PLANTS
24. TRANSGENICS IN CROP lMPROVEMENT
25. GENOMICS
26.BIOINFORMATICS
27.INTELLECTUAL PROPERTY RIGHTS
Appendix
References
Glossary
Author index
Subject index
